Introduction to Coelenterazine

Coelenterazine is a substrate for bioluminescent enzymes of marine origin like Renilla and Gaussia but also for Aequorin and Obelin.

Presently commercially available products have been found to contain less than 85% of the active substance.
However, both Coelenterazine and deoxy coelenterazine (benzyl-coelenterazine) supplied by RESEM are manufactured using newly developed methods, thus providing extremely pure and active luminophores without the need for extended chromatographic purification procedures.

An aqueous solution of coelenterazine should be freshly prepared, allowed to stabilize 15 minutes at room temperature and used within the same working day.
An alcoholic solution of coelenterazin can be kept in a refrigerator for as long as 3 weeks.
Coelenterazine as yellowish crystals should be stored in a dry and dark place under nitrogen or argon at -80ºC.

Shelf-life for unopened vials is 2 years after manufacturing.

Product Information Coelenterazine

Fig 1: Deoxy Coelenterazine (CLZ h)

Specifications for deoxy Coelenterazine (CLZ h)

- Appearance: powder with intensive yellow colour
- Stocking: at -20 to -80°C in dark and dryness
- Water content: <0.02%
- Chemical purity: >96% by HPLC
- Packed under argon, shipped refrigerated

Fig 2: Coelenterazine (nCLZ)

Specifications for Coelenterazine (nCLZ)

- Appearance: powder with intensive yellow colour
- Stocking: at -20 to -80°C in dark and dryness
- Water content: <0.02%
- Chemical purity: >95% by HPLC
- Packed under argon, shipped refrigerated