Product Information: D- & L-Luciferin

Product No. 260159 / 260201 / 260313 / 260401

QUALITY CONTROL PROCEDURES - Biochemical performance

The biochemical performance of D-luciferin is assayed at 25°C by an automatic procedure using a Berthold Luminometer. The assay is based on standardized preparations of all components (luciferase, ATP, buffer etc.) required for the measurement. The light emission values obtained with 1.000 and 0.250 mg/mL of D-Luciferin and compared with a reference lot of D-Luciferin.

Actual results for 5 samples from a typical lot, in the absence of substrate inhibition and non-competitive inhibitors, will approach or exceed 100%. With test and reference lots of similar qualities a value around 100% is expected.

Table 1: Biochemical performance of D-Luciferin

Reference is used in concentration of 0.200 mg/mL	Test 0.200 mg/mL
Lot No.1021	101.0 ± 1.9%
Specification	> 97.0%