Product Information: D- & L-Luciferin

Product No. 260159 / 260201

QUALITY CONTROL PROCEDURES - HPLC analysis of chemical purity

The analysis is performed with Shimadzu SPD-10A UV Spectrometric Detector in Shimadzu Liquid Chromatograph. The procedure uses a buffered-reverse phase technique with a gradient elution. An example of a chromatogram obtained with Lot No.1025 at 330 nm is shown in Fig. 1. Results from 5 samples performed in duplicates are summarized in Table 1. Results obtained at 265 nm were similar. In the interval 220-540 nm no additional chromatographic peaks were detected. The average retention time of D-Luciferin was 7.126±0.013 min.

Peak no.2 at 9.302±0.021 min corresponds to deoxyluciferin. The average percent purity for Lot No.1035 is 99.65±0.03% D-Luciferin. According to specifications, the percent purity of D-Luciferin (free acid) must be greater than 99.5%.

HPLC analysis of chemical purity

Fig. 1: Chromatogram of D-Luciferin (Lot No.1035) at 330 nm. Insert shows absorbance using an enlarged scale.

Table 1: Chemical purity analysed by HPLC. UV detection at 330 nm